XE49 pt Beta-catenin-myc
(Plasmid #16840)

• Depositing Lab: Randall Moon
• Publication: Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Backbone

• Vector backbone: pcS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pt beta-catenin-myc
• Alt name: beta catenin
• Species: X. laevis (frog)
• Mutation: four point mutations introduced into the GSK-3 phosphorylation site
• Entrez Gene: ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
• Tag / Fusion Protein:
  • 6x myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown.
The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation.

Ref: Yost et al., Genes & Development, 10:1443-1454, 1996.

four mutations are amino acids S33A, S37A, T41A, S45A