XE28 XBC 40
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16842||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4300
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namebeta catenin
SpeciesX. laevis (frog)
Insert Size (bp)2300
Entrez Genectnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
/ Fusion Protein
- 6x myc (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (unknown if destroyed)
- 3′ cloning site Cla1 (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed by David on date of 2/9/95 in book XVI p.106.
Notes for Use:
Full-length Xenopus beta-catenin reconstructed by taking the Cla-SphI vector fragment from XBC20 and the SphI-Cla sequences from XBC24 (encoding the C-terminus of beta-catenin). This corrects possible problems with the C-terminus os XBC20. To make RNA, cut with NotI and transcribe with SP6.
Ref: Yost et al. Genes and Development 10:1443-1454, 1996.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE28 XBC 40 was a gift from Randall Moon (Addgene plasmid # 16842 ; http://n2t.net/addgene:16842 ; RRID:Addgene_16842)
Map uploaded by the depositor.