XE220 mouse Wnt-1-pCS2P+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16850||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1500
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
- Cloning method Restriction Enzyme
- 5′ cloning site Stu1 (destroyed during cloning)
- 3′ cloning site Xba1 (destroyed during cloning)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Description: Full coding sequence of mouse Wnt-1, untagged. ~1.5kb insert isolated from Ajamete Kaykas's 2294 mWnt1 plasmid, Not1filled/Spe1 fragment cloned into pCS2P+ Stu1/Xba1.
Notes for Use: For in vitro transcription linearize with Asp718 and transcribe with SP6.
Reference: Clone made by Gilbert Weidinger, Moon lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE220 mouse Wnt-1-pCS2P+ was a gift from Randall Moon (Addgene plasmid # 16850 ; http://n2t.net/addgene:16850 ; RRID:Addgene_16850)