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XE47 dnWnt-8
(Plasmid #16858)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16858 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 16858-D50 50 μg of DNA in Tris buffer $495
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DNA 16858-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dnWnt-8
  • Alt name
    wnt8
  • Alt name
    wingless
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    794
  • Entrez Gene
    wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid History:
Constructed by Jeff Brown on date of 1/26/95.

Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.

This construct was generated by a PCR-mediated trunkation of pGEM Xwnt-8. Earlier versions of.
this construct known as X9spdyc21b and X8spdyc21c contained point mutations (notebook 2 & 3) by have.
activities identical to dnXwnt-8.

Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE47 dnWnt-8 was a gift from Randall Moon (Addgene plasmid # 16858 ; http://n2t.net/addgene:16858 ; RRID:Addgene_16858)

  • For your References section:

    Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos. Hoppler S, Brown JD, Moon RT. Genes Dev. 1996 Nov 1. 10(21):2805-17. 10.1101/gad.10.21.2805 PubMed 8946920
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