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XE46 dnWnt-1
(Plasmid #16860)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16860 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dnWnt-1
  • Alt name
    wnt-1
  • Alt name
    wnt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    915
  • Mutation
    truncated gene
  • Entrez Gene
    Wnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Plasmid History:
Constructed by Jeff Brown on date of 8/8/95.

Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.

PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD. The Wnt-1 gene is truncated after the SPNFC peptide sequence.

Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE46 dnWnt-1 was a gift from Randall Moon (Addgene plasmid # 16860 ; http://n2t.net/addgene:16860 ; RRID:Addgene_16860)