|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)915
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed by Jeff Brown on date of 8/8/95.
Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.
PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD. The Wnt-1 gene is truncated after the SPNFC peptide sequence.
Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE46 dnWnt-1 was a gift from Randall Moon (Addgene plasmid # 16860 ; http://n2t.net/addgene:16860 ; RRID:Addgene_16860)
Map uploaded by the depositor.