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(Plasmid #66106)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 66106 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 7484
  • Modifications to backbone
    The vector pCIG (pCAGGS-IRES2-nucEGFP) containing CMV enhancer/chicken b-actin promoter and rabbit b-globin polyA was double digested using ClaI and NotI to eliminate the IRES-nucGFP sequence.
  • Vector type
    Chicken expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
  • Entrez Gene
    FZD7 (a.k.a. Fz-7, cFz-7)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG Fzd7 GFP was a gift from Felicitas Proels & Martin Scaal (Addgene plasmid # 66106 ; ; RRID:Addgene_66106)
  • For your References section:

    Communication between distant epithelial cells by filopodia-like protrusions during embryonic development. Sagar, Prols F, Wiegreffe C, Scaal M. Development. 2015 Feb 15;142(4):665-71. doi: 10.1242/dev.115964. Epub 2015 Jan 23. 10.1242/dev.115964 PubMed 25617437