|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16925||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Entrez Genewnt1 (a.k.a. WNT-1, int-1, sb:eu647, zgc:194464, zgc:194478)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
cDNA clone of wnt-1 for DIG labelled probes cloned by G.
Kelly. Antisense: linearize with EcoR1 and transcribe with SP6 RNA polyermase. Sense: linearize with HindIII and transcribe with T7 RNA polymerase. Hydrolyze probes for 12 minutes according to Harland, 1991.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP16 Wnt-1 was a gift from Randall Moon (Addgene plasmid # 16925 ; http://n2t.net/addgene:16925 ; RRID:Addgene_16925)