p414GPD-3xFLAG-Nop4 WT
(Plasmid #169261)

• Purpose: S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT
• Depositing Lab: Susan Baserga
• Publication: Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017777118. doi: 10.1073/pnas.2017777118.

Backbone

• Vector backbone: p414GPD
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nop4 WT
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: NOP4 (a.k.a. NOP77)
• Promoter: GPD
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GACGGTAGGTATTGATTGTAATTCTGT
• 3′ sequencing primer: TTCGGTTAGAGCGGATGTGG

Resource Information

• Supplemental Documents:
  • p414GPD-3xFLAG-Nop4 WT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry