|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16932||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK+
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)2500
Entrez Geneactb1 (a.k.a. ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.
Constructed by Greg Kelly 5/91.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP9 Actin was a gift from Randall Moon (Addgene plasmid # 16932 ; http://n2t.net/addgene:16932 ; RRID:Addgene_16932)
Map uploaded by the depositor.