PA-Dre
(Plasmid
#169706)
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PurposePhotoactivatable Dre recombinase for AAV production and expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $34 USD for plasmid.
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How this works
- Place a request for a quantity of 2 (0.2 mL), 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
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- Receive your prep in 6–9 weeks after the MTA is approved by your organization.
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Backbone
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Vector backboneAdapted from px330
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDre246-nMag-T2A-pMag-Dre247
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SpeciesSynthetic
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Mutationnone
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer na
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PA-Dre was a gift from Dali Li (Addgene plasmid # 169706 ; http://n2t.net/addgene:169706 ; RRID:Addgene_169706) -
For your References section:
Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Li H, Zhang Q, Gu Y, Wu Y, Wang Y, Wang L, Feng S, Hu Y, Zheng Y, Li Y, Ye H, Zhou B, Lin L, Liu M, Yang H, Li D. Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33426-33435. doi: 10.1073/pnas.2003991117. Epub 2020 Dec 14. 10.1073/pnas.2003991117 PubMed 33318209