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Xenopus C-cadherin
(Plasmid #17023)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17023 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 17023-D50 50 μg of DNA in Tris buffer $495
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DNA 17023-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSP36T
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C-cadherin
  • Species
    X. laevis (frog)
  • Entrez Gene
    cdh3.S (a.k.a. XELAEV_18024919mg, E-Cad, E-cadherin, P-cadherin, U-cadherin, XB-cadherin, XBcad, XTCAD-1, b-cadherin, cadherin-1, cdh1-a, cdh3, cdh3-a, cdh3-b, cdhc-A, cdhp, hjmd, l-cam, lcam, pcad, uvo, uvomorulin, xb-cad, xcdh1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with EcoRI and transcribe with SP6.

A cDNA encoding Xenopus C-cadherin was cloned by screening a Xenopus lambda gt10 oocyte cDNA library. The cDNA was instered into pSP36T for transcription using the SP6 promoter.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xenopus C-cadherin was a gift from Barry Gumbiner (Addgene plasmid # 17023 ; http://n2t.net/addgene:17023 ; RRID:Addgene_17023)

  • For your References section:

    Disruption of gastrulation movements in Xenopus by a dominant-negative mutant for C-cadherin. Lee CH, Gumbiner BM. Dev Biol. 1995 Oct . 171(2):363-73. 10.1006/dbio.1995.1288 PubMed 7556920
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