|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17043||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namewnt-4 cDNA
SpeciesD. rerio (zebrafish)
Insert Size (bp)1300
Entrez Genewnt4a (a.k.a. wnt-4, wnt4, zgc:136737)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Insert: wnt-4 (zebrafish) cDNA.
Insert size: 1.3 Kb.
To make an in situ probe, linearize with EcoRV and transcribe with SP6.
To make sense RNA, linearize with Xba1 and transcribe with T7.
Constructed by Anne Ungar on date of 6/8/93.
Species of Sequence Origin: Zebrafish.
Notes for Use:
Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts.
Citation: Ungar et al., Mechanisms of Development 52 (1995)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE2 Wnt-4 was a gift from Randall Moon (Addgene plasmid # 17043 ; http://n2t.net/addgene:17043 ; RRID:Addgene_17043)
Map uploaded by the depositor.