ZE1 Wnt-1 myc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17042||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDoug Melton Lab (Addgene #15030)
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
SpeciesD. rerio (zebrafish)
Insert Size (bp)1200
Entrez Genewnt1 (a.k.a. WNT-1, int-1, sb:eu647, zgc:194464, zgc:194478)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Insert: wnt-1 myc.
Insert size: 1.2 Kb.
Transcription: linearize with Pst1, transcribe with Sp6.
Constructed by Greg Kelly on date of 1/27/93, page 20 Book 2.
Notes for Use:
There is also a construct where wnt-1 myc is clone in antisense orientation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE1 Wnt-1 myc was a gift from Randall Moon (Addgene plasmid # 17042 ; http://n2t.net/addgene:17042 ; RRID:Addgene_17042)