ZE19 wnt8 pBand3-5
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17053||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepGemT easy
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namewnt8 pBand3-5
SpeciesD. rerio (zebrafish)
Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
RT PCR prodct ligated into vector full length cDNA cloned includes ORF1, IRES and ORF2.
For antiense RNA: transcribe with SP6 polymerase.
Species of Sequence Origin: Zebrafish.
Reference: Lekven, December, Cell, 2001.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE19 wnt8 pBand3-5 was a gift from Randall Moon (Addgene plasmid # 17053 ; http://n2t.net/addgene:17053 ; RRID:Addgene_17053)
Map uploaded by the depositor.