|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17044||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namewnt-8 full length
SpeciesD. rerio (zebrafish)
Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Insert: wnt-8 full length.
Transcription: linearize at EcoR1, transcribe with T7.
Constructed by Greg Kelly on date of 2/10/94.
Species of Origin: Zebrafish.
Note for Use:
Note: Insert was isolated using PCR, there is not pA trail and very little 5' or 3' UTR of the wnt-8 cDNA.
Citation: Kelly et al., Development 121 1787 (1995)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE3 Wnt-8 was a gift from Randall Moon (Addgene plasmid # 17044 ; http://n2t.net/addgene:17044 ; RRID:Addgene_17044)
Map uploaded by the depositor.