ZE3 Wnt-8
(Plasmid #17044)

• Depositing Lab: Randall Moon
• Publication: Zebrafish Expression Plasmids (unpublished)

Backbone

• Vector backbone: pT7Ts
• Backbone size w/o insert (bp): 3000
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: wnt-8 full length
• Alt name: wnt8
• Species: D. rerio (zebrafish)
• Entrez Gene: wnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: wnt-8 full length.
Transcription: linearize at EcoR1, transcribe with T7.

Plasmid History:
Constructed by Greg Kelly on date of 2/10/94.
Species of Origin: Zebrafish.

Note for Use:
Note: Insert was isolated using PCR, there is not pA trail and very little 5' or 3' UTR of the wnt-8 cDNA.

Citation: Kelly et al., Development 121 1787 (1995)