pYY_S1_400
(Plasmid
#170470)
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PurposeTo amplify transcription template containing LacI operators
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT7A1, Os, O1, lambda t1, in lacZ fragment
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SpeciesSynthetic
- Promoter T7A1
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LacI operators separated by 400 base pairs.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYY_S1_400 was a gift from Laura Finzi (Addgene plasmid # 170470 ; http://n2t.net/addgene:170470 ; RRID:Addgene_170470) -
For your References section:
Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Interactions with Transcription Factors. Kovari DT, Yan Y, Finzi L, Dunlap D. Methods Mol Biol. 2018;1665:317-340. doi: 10.1007/978-1-4939-7271-5_17. 10.1007/978-1-4939-7271-5_17 PubMed 28940077
