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SIN40C.SFFV.dCas9-KRAB.P2A.mNeon
(Plasmid #170482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170482 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    SIN40C
  • Total vector size (bp) 12435
  • Modifications to backbone
    modified pLKO.005
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9-KRAB
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    4482
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    SFFV
  • Alt name
    Spleen Focus Forming Virus Promoter Enhancer
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    413

Gene/Insert 3

  • Gene/Insert name
    P2A-mNeonGreen
  • Species
    Synthetic
  • Insert Size (bp)
    780

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIN40C.SFFV.dCas9-KRAB.P2A.mNeon was a gift from Dirk Heckl (Addgene plasmid # 170482 ; http://n2t.net/addgene:170482 ; RRID:Addgene_170482)

  • For your References section:

    Myeloid leukemia vulnerabilities embedded in long noncoding RNA locus MYNRL15. Ng M, Verboon L, Issa H, Bhayadia R, Vermunt MW, Winkler R, Schuler L, Alejo O, Schuschel K, Regenyi E, Borchert D, Heuser M, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH. iScience. 2023 Sep 7;26(10):107844. doi: 10.1016/j.isci.2023.107844. eCollection 2023 Oct 20. 10.1016/j.isci.2023.107844 PubMed 37766974
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