pUC19-eGFP-P2A-dTAG-Ing3
(Plasmid
#170754)
-
PurposeDonor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus
-
Depositing Lab
-
Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 170754-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 170754-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 5196
-
Vector typeMouse Targeting
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeGFP-P2A-2xHA-dTAG
-
Alt nameeGFP-P2A-2xHA-FKBP12(F36V)
-
SpeciesM. musculus (mouse), Synthetic
-
Insert Size (bp)2400
-
MutationPhenylalanine 36 to valine in FKBP12
- Promoter none
-
Tag
/ Fusion Protein
- eGFP-P2A-2xHA-dTAG
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13/pUC Reverse
- 3′ sequencing primer M13/pUC Forward
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bythe P2A-2xHA-dTAG cassette was amplified by PCR from pCRIS-PITChv2-BSD-dTAG BRD4 vector (addgene #91792)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 170754-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 170754-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUC19-eGFP-P2A-dTAG-Ing3 was a gift from Kristian Helin (Addgene plasmid # 170754 ; http://n2t.net/addgene:170754 ; RRID:Addgene_170754) -
For your References section:
Generation of locus-specific degradable tag knock-ins in mouse and human cell lines. Damhofer H, Radzisheuskaya A, Helin K. STAR Protoc. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575. eCollection 2021 Jun 18. 10.1016/j.xpro.2021.100575 PubMed 34151298
Map uploaded by the depositor.
