3xFlag-HaloTag-EZH2 HDR - pDY053
(Plasmid
#171108)
-
PurposeHomology directed repair plasmid for generating HaloTag-EZH2 cells
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepuc19
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEZH2
-
SpeciesH. sapiens (human)
-
Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
3xFlag-HaloTag-EZH2 HDR - pDY053 was a gift from Thomas Cech (Addgene plasmid # 171108 ; http://n2t.net/addgene:171108 ; RRID:Addgene_171108) -
For your References section:
Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits. Youmans DT, Schmidt JC, Cech TR. Genes Dev. 2018 Jun 1;32(11-12):794-805. doi: 10.1101/gad.311936.118. Epub 2018 Jun 11. 10.1101/gad.311936.118 PubMed 29891558
Map uploaded by the depositor.
