pTetR-LasR-pLuxR-GFP
(Plasmid #171159)

• Purpose: Sensing Device with Reporter protein
• Depositing Lab: Matthew Chang
• Publication: Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 10.1038/msb.2011.55.

Backbone

• Vector backbone: pSB1A2
• Backbone size w/o insert (bp): 2079
• Total vector size (bp): 3966
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: lasR
• Alt name: transcriptional regulator LasR
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 723
• Promoter: pTet

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: Nil
• 3′ sequencing primer: Nil

Gene/Insert 2

• Gene/Insert name: gfp
• Alt name: green fluorescence protein GFP
• Species: Aequorea victoria
• Insert Size (bp): 717
• Promoter: pLux

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: Nil
• 3′ sequencing primer: Nil

Resource Information

• Supplemental Documents:
  • pSB1A2-pTet-r5-LasR-T-pLux-r3-GFP-T.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry