pKAR2-Br512g3.1
(Plasmid
#171200)
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PurposeExpresses Br512g3.1 polymerase in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171200 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKAR2
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Backbone manufacturerIn-house made
- Backbone size w/o insert (bp) 5187
- Total vector size (bp) 6218
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEngineered Bst DNA Polymerase I Large Fragment
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Alt nameBr512g3.1
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SpeciesSynthetic; Geobacillus stearothermophilus
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Insert Size (bp)1932
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GenBank ID4O0I_A
- Promoter T7
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Tag
/ Fusion Protein
- 8x His tag; Villin Headpiece 47 amino acid (vHP47, supercharged) ; Linker (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer CCTATAAAAATAGGCGTATCACGAGGC
- 3′ sequencing primer GTGTAACGACCGGCTAGAATCACTCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.15.439918v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKAR2-Br512g3.1 was a gift from Andrew Ellington (Addgene plasmid # 171200 ; http://n2t.net/addgene:171200 ; RRID:Addgene_171200)