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Addgene

pGTPA
(Plasmid #171525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171525 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACRISPR
  • Vector type
    CRISPR
  • Selectable markers
    Carbenicillin; 50 ug/mL in E.coli and 150 ug/mL in Pseudomonas aeruginosa

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas12k gRNA, transposon donor cassette
  • gRNA/shRNA sequence
    Unpsecified
  • Species
    Synthetic
  • Promoter Constitutive promoter J23119

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTPA was a gift from Quanjiang Ji (Addgene plasmid # 171525 ; http://n2t.net/addgene:171525 ; RRID:Addgene_171525)

  • For your References section:

    Targeted genetic screening in bacteria with a Cas12k-guided transposase. Chen W, Ren ZH, Tang N, Chai G, Zhang H, Zhang Y, Ma J, Wu Z, Shen X, Huang X, Luo GZ, Ji Q. Cell Rep. 2021 Aug 31;36(9):109635. doi: 10.1016/j.celrep.2021.109635. 10.1016/j.celrep.2021.109635 PubMed 34469724
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