M18 pCS-twhh-nBeta gal Vec
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17155||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCS-NBeta gal
Growth in Bacteria
Gene/Insert nametwhh promoter
Alt nametiggy-winkle hedgehog
SpeciesD. rerio (zebrafish)
Insert Size (bp)5200
Entrez Geneshhb (a.k.a. twh, twhh)
/ Fusion Protein
- nuclear beta-gal (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
Parent vector: pCS-twhh-nBeta gal.
Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb)
Notes for Use:
To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence.
Citation: Du et al., 1997, J. Cell Biology.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M18 pCS-twhh-nBeta gal Vec was a gift from Randall Moon (Addgene plasmid # 17155 ; http://n2t.net/addgene:17155 ; RRID:Addgene_17155)
Map uploaded by the depositor.