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pαH-S-GSAS-B.1.1.7
(Plasmid #171747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171747 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 171747-D50 50 μg of DNA in Tris buffer $495
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DNA 171747-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (S-GSAS-B.1.1.7 variant)
  • Alt name
    UK SARS-CoV-2 spike variant
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3615
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; D614G mutation; H69/V70del; Y144del; N501Y; A570D; P681H; T716I; S982A; D1118H
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • HRV3C cleavage site, 8X His tag, 2X Strep-Tag II (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-GSAS-B.1.1.7 was a gift from Priyamvada Acharya (Addgene plasmid # 171747 ; http://n2t.net/addgene:171747 ; RRID:Addgene_171747)

  • For your References section:

    Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Stalls V, Kopp MF, Manne K, Li D, Wiehe K, Saunders KO, Edwards RJ, Korber B, Haynes BF, Henderson R, Acharya P. Science. 2021 Jun 24. pii: science.abi6226. doi: 10.1126/science.abi6226. 10.1126/science.abi6226 PubMed 34168071
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