pRN3P_T3_BE4_IVT
(Plasmid #171762)

• Purpose: Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase
• Depositing Lab: Timo Otonkoski
• Depositing Organization: University of Helsinki
  Located in Finland
• Publication: Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S2213-6711(21)00552-X. doi: 10.1016/j.stemcr.2021.10.017.

Backbone

• Vector backbone: pRN3P
• Backbone manufacturer: Torres-Padilla et al., 2007
• Backbone size w/o insert (bp): 3228
• Total vector size (bp): 8706
• Vector type: Vector for in-vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Grow solid and liquid cultures always at 30°C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BE4
• Alt name: Cytosine Base Editor
• Species: Synthetic
• Insert Size (bp): 5478
• Promoter: T3 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamhI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: BE4 was a gift from David Liu (Addgene plasmid # 100802 ; http://n2t.net/addgene:100802 ; RRID:Addgene_100802). The in-vitro transcription backbone was published by Torres-Padilla et al., 2007.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence differs from the depositor reference sequence. These differences do not affect plasmid function. Compared the .gb file linked above, the Addgene sequence shows there are deletions in the MCS/Ruby (229-239 bp), poly C 3'UTR region (5952-5956 bp), and backbone.

DNA (Catalog # 171762-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 171762-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.