M63 pPET28a-TEV-human beta cat domain C(666-781)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17204||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepET-28 a
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert namebeta catenin
SpeciesH. sapiens (human)
Insert Size (bp)345
Mutationcarboxy-terminal residues 666-781
Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
/ Fusion Protein
- HIS (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI / SalI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Description: This is an amino terminal HIS tag, then TEV protease site, then human beta catenin carboxy terminal residues 666-781, as a BamHI-XhoI fragment cloned into BamHI and SalI sites.
Reference: Made by Ken-Ichi Takemaru.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M63 pPET28a-TEV-human beta cat domain C(666-781) was a gift from Randall Moon (Addgene plasmid # 17204 ; http://n2t.net/addgene:17204 ; RRID:Addgene_17204)