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pAd-PV-NES-DsRed
(Plasmid #17286)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17286 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Adenoviral Recombinant (pAdEasy)
  • Backbone size w/o insert (bp) 33400
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PV-NES
  • Alt name
    PV-NES-DsRed-myc
  • Alt name
    Parvalbumin
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1100
  • GenBank ID
    M12725
  • Entrez Gene
    Pvalb (a.k.a. PALB1, Pva)
  • Tags / Fusion Proteins
    • DSR (C terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GTGGCAAAAGTGACGTTTTTGG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

AdEasy is a registered trademark of the Johns Hopkins University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-PV-NES-DsRed was a gift from Anton Bennett (Addgene plasmid # 17286 ; http://n2t.net/addgene:17286 ; RRID:Addgene_17286)
  • For your References section:

    Nucleoplasmic calcium is required for cell proliferation. Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC, Bennett AM, Nathanson MH. J Biol Chem. 2007 Jun 8. 282(23):17061-8. 10.1074/jbc.M700490200 PubMed 17420246