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pGlomyc-Klf4
(Plasmid #172870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172870 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 172870-D50 50 μg of DNA in Tris buffer $495
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DNA 172870-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGlomyc (based on pCDNA3.1)
  • Backbone manufacturer
    Olivier Destree
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6400
  • Modifications to backbone
    The pGlomyc vector backbone contains 5’ and 3’ globin UTRs and the pCDNA1.1 BamHI-NsiI polylinker in the backbone of pCDNA3.1(+).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Klf4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1452
  • Entrez Gene
    Klf4 (a.k.a. EZF, Gklf, Zie)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene sequence was amplified by PCR in our lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGlomyc-Klf4 was a gift from Renee van Amerongen (Addgene plasmid # 172870 ; http://n2t.net/addgene:172870 ; RRID:Addgene_172870)

  • For your References section:

    Hyperactive WNT/CTNNB1 signaling induces a competing cell proliferation and epidermal differentiation response in the mouse mammary epithelium. Mourao L, Zeeman AL, Wiese KE, Bongaarts A, Oudejans LL, Martinez IM, van de Grift YBC, Jonkers J, van Amerongen R. bioRxiv 2021.06.22.449461 10.1101/2021.06.22.449461
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