PurposeDead SNAP tag at the centrosome
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||173073||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4091
- Total vector size (bp) 6686
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namepericentrin AKAP450 centrosomal-targeting domain
SpeciesH. sapiens (human)
Insert Size (bp)2627
MutationAA144 in both SNAP tags changed from a cysteine to an alanine
- Promoter CMV IE94
/ Fusion Protein
- mCherry-SNAP-SNAP-Varied (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byJohn Scott Lab, University of Washington, Seattle WA
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The SNAP gene/insert was a gift from the Scott lab as part of collaboration on the manuscript entitled "Subcellular drug targeting illuminates local kinase action"
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-mCherry_SNAP_SNAPV_PACT_Dead was a gift from Heidi Hehnly (Addgene plasmid # 173073 ; http://n2t.net/addgene:173073 ; RRID:Addgene_173073)
For your References section:Subcellular drug targeting illuminates local kinase action. Bucko PJ, Lombard CK, Rathbun L, Garcia I, Bhat A, Wordeman L, Smith FD, Maly DJ, Hehnly H, Scott JD. Elife. 2019 Dec 24;8. pii: 52220. doi: 10.7554/eLife.52220. 10.7554/eLife.52220 PubMed 31872801