pCS2-mCherry_SNAP_SNAPV_PACT_Dead
(Plasmid #173073)

• Purpose: Dead SNAP tag at the centrosome
• Depositing Lab: Heidi Hehnly
• Publication: Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 10.7554/eLife.52220.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4091
• Total vector size (bp): 6686
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-SNAP-SNAP-Varied-PACT
• Alt name: pericentrin AKAP450 centrosomal-targeting domain
• Species: H. sapiens (human)
• Insert Size (bp): 2627
• Mutation: AA144 in both SNAP tags changed from a cysteine to an alanine
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • mCherry-SNAP-SNAP-Varied (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: John Scott Lab, University of Washington, Seattle WA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The SNAP gene/insert was a gift from the Scott lab as part of collaboration on the manuscript entitled "Subcellular drug targeting illuminates local kinase action"