DPY-pNCS
(Plasmid #17365)

• Depositing Lab: Stephen Goff
• Publication: Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Backbone

• Vector backbone: pBR322 modified to include SV40 origin
• Backbone size w/o insert (bp): 2000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MMLV proviral DNA
• Species: Moloney murine leukemia virus
• Insert Size (bp): 8000
• Mutation: deletion that removes 5 amino acids (DPPPY) from p12 region

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pNCS contains an infectious copy of M-MuLV proviral DNA and carries a simian virus 40 replication origin to allow high-level expression in 293T cells. DPY-pNCS is similar to the wild-type parent but contains a deletion that removes 5 amino acids (DPPPY) from p12 region.