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Addgene

pcDNA3 HA-Sumo1 WT
(Plasmid #48966)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48966 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 327
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    327
  • Entrez Gene
    SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
  • Promoter T7
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA-Sumo1 WT was a gift from Guy Salvesen (Addgene plasmid # 48966 ; http://n2t.net/addgene:48966 ; RRID:Addgene_48966)
  • For your References section:

    The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS. J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19. 10.1074/jbc.M110.205153 PubMed 21247896