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pBID2_Gateway>QF2
(Plasmid #173728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173728 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBID2
  • Backbone manufacturer
    Evelyne Ruchti and Brian McCabe
  • Backbone size (bp) 11967
  • Vector type
    Insect Expression ; Gateway Destination vector
  • Selectable markers
    Drosophila mini-white

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    QF2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF2 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBID2_Gateway>QF2 was a gift from Brian McCabe (Addgene plasmid # 173728 ; http://n2t.net/addgene:173728 ; RRID:Addgene_173728)
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