PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||174100||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4474
- Total vector size (bp) 5734
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberLow Copy
Gene/Insert nameAcridone 82 tRNA synthetase and tRNA
Insert Size (bp)1260
MutationN311S, C313G, V366A, W382T
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDule2-Acd82 was a gift from Ryan Mehl (Addgene plasmid # 174100 ; http://n2t.net/addgene:174100 ; RRID:Addgene_174100)
For your References section:Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells. Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS, Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson JE. Chem. Sci., 2021,12, 11955-11964 10.1039/D1SC01914G