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Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells.
Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS, Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson EJ
Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. eCollection 2021 Sep 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174099pDule-Acd82Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
174100pDule2-Acd82Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
174101pAcBac1.tR4-AcdRS82Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Antibodies from Article