pDule-Acd82
(Plasmid #174099)

• Purpose: Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
• Depositing Lab: Ryan Mehl
• Publication: Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. eCollection 2021 Sep 22.

Backbone

• Vector backbone: pDule
• Backbone size w/o insert (bp): 3801
• Total vector size (bp): 5061
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Acridone 82 tRNA synthetase and tRNA
• Species: Methanosarcina barkeri
• Insert Size (bp): 1260
• Mutation: N311S, C313G, V366A, W382T

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry