|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17419||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2581
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMET3 promoter + polylinker + CYC1 terminator
Alt nameATP sulfurylase
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2413
MutationMET3 promoter is between SacI and XbaI restriction sites.
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site BglI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPlasmid was created by Paula Calle. Insert was cut from p405MET3.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p404MET3 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17419 ; http://n2t.net/addgene:17419 ; RRID:Addgene_17419)