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Anti-Halobacterium GvpA scFv 2B10-1-pcDNA 3.4 TOPO
(Plasmid #174688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174688 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA 3.4 TOPO
  • Backbone manufacturer
    Sigma Aldrich
  • Backbone size w/o insert (bp) 6011
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses Expi293F human cells for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti- Halobacterium salinarum GvpA single chain variable fragment 2B10-1
  • Alt name
    anti-GvpA scfv
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    798
  • Mutation
    None
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • hemagglutinin (HA) tag, hexahistidine (HIS) tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Halobacterium GvpA scFv 2B10-1-pcDNA 3.4 TOPO was a gift from Jean Gariepy (Addgene plasmid # 174688 ; http://n2t.net/addgene:174688 ; RRID:Addgene_174688)

  • For your References section:

    Broadly Applicable Bispecific Linker Approach to Noncovalently Target Therapeutic Nanoparticles to Tumor Cells Expressing Carcinoembryonic Antigen. Fernando A, Sparkes A, Matus EI, Patel A, Foster FS, Goertz D, Lee P, Gariepy J. ACS Pharmacol Transl Sci. 2024 Jun 3;7(6):1864-1873. doi: 10.1021/acsptsci.4c00140. eCollection 2024 Jun 14. 10.1021/acsptsci.4c00140 PubMed 38898951
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