Affibody EGFR:1907.BirA*-pET301/NT-DEST
(Plasmid #174691)

• Purpose: Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*
• Depositing Lab: Jean Gariepy
• Publication: Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2):62-69. doi: 10.1021/acsptsci.1c00192. eCollection 2022 Feb 11.

Backbone

• Vector backbone: pET301/NT-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7382
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The depositing lab recommends BL21(DE3) cells for bacterial expression.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
• Alt name: Anti EGFR affibody.BirA*
• Species: Staphylococcus aureus and Escherichia coli
• Insert Size (bp): 1237
• Mutation: R118G mutation in BirA
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • HIS tag (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry