pRM16
(Plasmid
#174787)
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PurposeConstitutive expression of the λCI protein; used as three-hybrid control corresponding to pCW17 (addgene #174664)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepACYC184
- Total vector size (bp) 3159
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone - empty vector
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Tag
/ Fusion Protein
- λCI
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRM16 was a gift from Katherine Berry (Addgene plasmid # 174787 ; http://n2t.net/addgene:174787 ; RRID:Addgene_174787) -
For your References section:
A bacterial three-hybrid assay for forward and reverse genetic analysis of RNA-protein interactions. Stockert OM, Gravel CM, Berry KE. Nat Protoc. 2022 Apr;17(4):941-961. doi: 10.1038/s41596-021-00657-4. Epub 2022 Feb 23. 10.1038/s41596-021-00657-4 PubMed 35197605