pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
(Plasmid #175018)

• Purpose: Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP
• Depositing Lab: Robert de Vries
• Publication: Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v13040586. doi: 10.3390/v13040586.

Backbone

• Vector backbone: pCD5
• Backbone manufacturer: -
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: OK-HEF s57a ED
• Species: Synthetic
• Mutation: s57a esterase knock out mutant
• GenBank ID: MG720235
• Promoter: CMV
• Tag / Fusion Protein:
  • GCN4-TEV-sfGFP-TwinStrep (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: GGC TAA CTA GAG AAC CCA CTG C
• 3′ sequencing primer: TTC AGG GGG AGG TGT GGG AG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry