pcDNA3.1(+)_Pmel-1_TCRB
(Plasmid #175288)

• Purpose: Expresses Pmel-1 TCR beta chain
• Depositing Lab: Jeff Hammerbacher
• Publication: Gottschalk et al bioRxiv 2021.11.30.470597

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6318
• Vector type: Mammalian Expression ; Suitable for IVT
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T cell receptor beta chain
• Alt name: T cell receptor beta chain mRNA, complete cds
• Species: M. musculus (mouse)
• Insert Size (bp): 954
• GenBank ID: EF154514.1
• Entrez Gene: Tcrb (a.k.a. TCRbeta, Tib)
• Promoter: CMV and T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+)_Pmel_TCRB.gb
• A portion of this plasmid was derived from a plasmid made by: The vector was from Genscript. The insert sequence was taken from GenBank (EF154514.1). Reference cited below in comments:

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Overwijk, Willem W., Marc R. Theoret, Steven E. Finkelstein, Deborah R. Surman, Laurina A. de Jong, Florry A. Vyth-Dreese, Trees A. Dellemijn, et al. 2003. “Tumor Regression and Autoimmunity after Reversal of a Functionally Tolerant State of Self-Reactive CD8+ T Cells.” The Journal of Experimental Medicine 198 (4): 569–80.