Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17567||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTLX2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)1700
Entrez GeneTlx2 (a.k.a. Enx, NCX, Ncx1, Tlx1l1, Tlx1l2, Hox11l1, Hox11L.1)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TLX2-lux was a gift from Jeff Wrana (Addgene plasmid # 17567 ; http://n2t.net/addgene:17567 ; RRID:Addgene_17567)
For your References section:The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Tang SJ, Hoodless PA, Lu Z, Breitman ML, McInnes RR, Wrana JL, Buchwald M. Development. 1998 May . 125(10):1877-87. PubMed 9550720
Map uploaded by the depositor.