|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24643||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5597
Modifications to backbone3ARE + TATA box
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer LucNRev (Common Sequencing Primers)
Took insert containing 3ARE + TATA box from A3-CAT using blunted HindIII and XhoI into pGL2-basic with a blunted EcoRI and XhoI. ARE sequence - TATCTGCTGCCCTAAAATGTGTATTCCATGGAAATGTCTGCCCTTCTCTC
NB: The A30-CAT map is attached, NOT the map for this plasmid (AR3-Lux)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAR3-Lux was a gift from Jeff Wrana (Addgene plasmid # 24643 ; http://n2t.net/addgene:24643 ; RRID:Addgene_24643)
Map uploaded by the depositor.