DR.GFP
(Plasmid #17617)

• Depositing Lab: Linzhao Cheng
• Publication: Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Backbone

• Vector backbone: SIN LV pRLLhPGK.GFP Sin-18 (modified)
• Backbone manufacturer: Dr. Didier Trono
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HLA-DRa promoter
• Alt name: (MHC II) HLA-DR alpha promoter
• Species: H. sapiens (human)
• Insert Size (bp): 300
• GenBank ID: X00274
• Entrez Gene: HLA-DRA (a.k.a. HLA-DRA1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Annotated Sequence
• A portion of this plasmid was derived from a plasmid made by: SIN LV pRLLhPGK.GFP Sin-18 (abbreviated as PGK.GFP) was kindly provided by Dr. Didier Trono.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs).

We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells.

Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.