|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14689||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePECAM promoter/enhancer
SpeciesM. musculus (mouse)
Insert Size (bp)8000
Entrez GenePecam1 (a.k.a. C85791, Cd31, PECAM-1, Pecam)
/ Fusion Protein
- H2B-GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PECAM-H2B-GFP was a gift from Victoria Bautch (Addgene plasmid # 14689 ; http://n2t.net/addgene:14689 ; RRID:Addgene_14689)
For your References section:Orientation of endothelial cell division is regulated by VEGF signaling during blood vessel formation. Zeng G, Taylor SM, McColm JR, Kappas NC, Kearney JB, Williams LH, Hartnett ME, Bautch VL. Blood. 2007 Feb 15. 109(4):1345-52. 10.1182/blood-2006-07-037952 PubMed 17068148
Map uploaded by the depositor.