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PECAM-H2B-GFP
(Plasmid #14689)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14689 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EGFP-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PECAM promoter/enhancer
  • Alt name
    PECAM
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8000
  • Entrez Gene
    Pecam1 (a.k.a. C85791, Cd31, PECAM-1, Pecam)
  • Tag / Fusion Protein
    • H2B-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H. Scott Baldwin, Vanderbilt

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PECAM-H2B-GFP was a gift from Victoria Bautch (Addgene plasmid # 14689 ; http://n2t.net/addgene:14689 ; RRID:Addgene_14689)
  • For your References section:

    Orientation of endothelial cell division is regulated by VEGF signaling during blood vessel formation. Zeng G, Taylor SM, McColm JR, Kappas NC, Kearney JB, Williams LH, Hartnett ME, Bautch VL. Blood. 2007 Feb 15. 109(4):1345-52. 10.1182/blood-2006-07-037952 PubMed 17068148