pmr101A-hVPS26
(Plasmid #17636)

• Depositing Lab: James Hurley
• Publication: Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):540-8.

Backbone

• Vector backbone: modified pmr101
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 4100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: VPS26
• Species: H. sapiens (human)
• Entrez Gene: VPS26A (a.k.a. HB58, Hbeta58, PEP8A, VPS26)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI? (see comments) (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR was used to amplify the cDNA coding for human Vps26A and to add a C-terminal His5 tag (GLVPRGSHHHHH). The expressed recombinant protein contains the additional dipeptide MetGly at the N terminus as a result of the vector construction.

Note: the publication lists NdeI as the 5' prime cloning site; however, this site is not unique.