Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #17636)


Item Catalog # Description Quantity Price (USD)
Plasmid 17636 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pmr101
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    VPS26A (a.k.a. HB58, Hbeta58, PEP8A, VPS26)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI? (see comments) (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR was used to amplify the cDNA coding for human Vps26A and to add a C-terminal His5 tag (GLVPRGSHHHHH). The expressed recombinant protein contains the additional dipeptide MetGly at the N terminus as a result of the vector construction.

Note: the publication lists NdeI as the 5' prime cloning site; however, this site is not unique.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmr101A-hVPS26 was a gift from James Hurley (Addgene plasmid # 17636 ; ; RRID:Addgene_17636)
  • For your References section:

    The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Shi H, Rojas R, Bonifacino JS, Hurley JH. Nat Struct Mol Biol. 2006 Jun . 13(6):540-8. 10.1038/nsmb1103 PubMed 16732284