|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17636||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified pmr101
- Backbone size w/o insert (bp) 4100
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Entrez GeneVPS26A (a.k.a. HB58, Hbeta58, PEP8A, VPS26)
/ Fusion Protein
- His (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI? (see comments) (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
PCR was used to amplify the cDNA coding for human Vps26A and to add a C-terminal His5 tag (GLVPRGSHHHHH). The expressed recombinant protein contains the additional dipeptide MetGly at the N terminus as a result of the vector construction.
Note: the publication lists NdeI as the 5' prime cloning site; however, this site is not unique.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmr101A-hVPS26 was a gift from James Hurley (Addgene plasmid # 17636 ; http://n2t.net/addgene:17636 ; RRID:Addgene_17636)
For your References section:The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Shi H, Rojas R, Bonifacino JS, Hurley JH. Nat Struct Mol Biol. 2006 Jun . 13(6):540-8. 10.1038/nsmb1103 PubMed 16732284