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pmr101A-hVPS26
(Plasmid #17636)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17636 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pmr101
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VPS26
  • Species
    H. sapiens (human)
  • Entrez Gene
    VPS26A (a.k.a. HB58, Hbeta58, PEP8A, VPS26)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI? (see comments) (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

PCR was used to amplify the cDNA coding for human Vps26A and to add a C-terminal His5 tag (GLVPRGSHHHHH). The expressed recombinant protein contains the additional dipeptide MetGly at the N terminus as a result of the vector construction.

Note: the publication lists NdeI as the 5' prime cloning site; however, this site is not unique.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmr101A-hVPS26 was a gift from James Hurley (Addgene plasmid # 17636 ; http://n2t.net/addgene:17636 ; RRID:Addgene_17636)
  • For your References section:

    The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Shi H, Rojas R, Bonifacino JS, Hurley JH. Nat Struct Mol Biol. 2006 Jun . 13(6):540-8. 10.1038/nsmb1103 PubMed 16732284