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Hm-tnn::TagRFP-T
(Plasmid #176425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176425 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCRII-TOPO plasmid
  • Vector type
    Transgenesis in Hofstenia miamia

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tagRFP-T
  • Promoter Hofstenia miamia Troponin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (unknown if destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer gcgatcgcTCATATGCTGTGGAGGTTAATCTGT
  • 3′ sequencing primer ggcgcgccCTAAAAATTCAATTATTTCATTT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hm-tnn::TagRFP-T was a gift from Mansi Srivastava (Addgene plasmid # 176425 ; http://n2t.net/addgene:176425 ; RRID:Addgene_176425)

  • For your References section:

    Transgenesis in the acoel worm Hofstenia miamia. Ricci L, Srivastava M. Dev Cell. 2021 Nov 22;56(22):3160-3170.e4. doi: 10.1016/j.devcel.2021.10.012. Epub 2021 Nov 8. 10.1016/j.devcel.2021.10.012 PubMed 34752780
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