Skip to main content
Addgene
Open Menu

Hm-epiA::TagRFP-T
(Plasmid #176430)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176430 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pCRII-TOPO plasmid
  • Vector type
    Transgenesis in Hofstenia miamia

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tagRFP-T
  • Promoter Hofstenia miamia epiA gene promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (destroyed during cloning)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer gcgatcgcTACCTACTCTTGCACCCCTTTATA
  • 3′ sequencing primer gcgatcgcTGCACTATCCTAACTACAACAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hm-epiA::TagRFP-T was a gift from Mansi Srivastava (Addgene plasmid # 176430 ; http://n2t.net/addgene:176430 ; RRID:Addgene_176430)

  • For your References section:

    Transgenesis in the acoel worm Hofstenia miamia. Ricci L, Srivastava M. Dev Cell. 2021 Nov 22;56(22):3160-3170.e4. doi: 10.1016/j.devcel.2021.10.012. Epub 2021 Nov 8. 10.1016/j.devcel.2021.10.012 PubMed 34752780
Related items: