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(Plasmid #17672)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17672 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI / BglII (destroyed during cloning)
  • 3′ cloning site BamHI / BglII (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMcsrc was constructed by gel purifying the 8.1-kilobase (kb) BamHI c-src fragment running from c-src coordinates -2.1 to 6.0 kb and ligating it into plasmid pEVX which had been linearized at a unique BglII site lying between the two Moloney murine leukemia virus (MoMLV) long terminal
repeats (LTRs). The upstream BamHI site is about 270 base
pairs (bp) upstream from the beginning of c-src exon no. 2, whereas the downstream site lies about 1.2 kb beyond the c-src termination codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMcSrc was a gift from David Shalloway (Addgene plasmid # 17672 ; ; RRID:Addgene_17672)
  • For your References section:

    Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells. Johnson PJ, Coussens PM, Danko AV, Shalloway D. Mol Cell Biol. 1985 May . 5(5):1073-83. PubMed 2582237