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pYTRW10K_0x5
(Plasmid #177278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177278 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    yTREX backbone
  • Backbone size (bp) 9699
  • Vector type
    Bacterial Expression, Synthetic Biology ; Yeast Expression, Tn5 genomic integration
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Part of pYT vector series which enables chromosomal integration via random transposition, facilitated by transposon Tn5 elements. Basic vector carrying the "empty" YT_core with specific cloning slots separated by designated upstream and downstream sequences for integron customization. Target genes can be inserted at the I-SceI site; additional marker, reporter or promoter elements may be integrated at further positions. Vector backbone is based on the yTREX vector and is equipped with the bacterial pMB1 ori, aphII-encoded KmR and mob gene from pBBR1 including promoter and oriT region and with yeast replication elements CEN4-ARS1 and URA3 marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYTRW10K_0x5 was a gift from Karl-Erich Jaeger (Addgene plasmid # 177278 ; http://n2t.net/addgene:177278 ; RRID:Addgene_177278)

  • For your References section:

    The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies S, Loeschcke A. FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. eCollection 2023. 10.1093/femsmc/xtac030 PubMed 37333445
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